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            熱門關鍵詞:tct耗材液基細胞處理試劑盒代理病理組織標本固定劑環保組織固定液

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            液基細胞處理試劑盒小常識

            文章出處:責任編輯:作者:人氣:-發表時間:2014-07-30 09:47:00【

             

             

                 中山市康乃欣生物醫療科技有限公司生產的液基細胞試劑盒主要是用于宮頸脫落細胞、尿液、腦脊液、痰液、穿刺液、胸腹水等各種液基細胞標本的檢查及診斷,但現主要廣泛應用于宮頸癌篩查。由于不同科室叫法不一,用于婦科TCT檢查的有的叫宮頸癌篩查試劑盒;宮頸癌篩查耗材,宮頸癌篩查試劑等。而用于婦科脫落細胞檢查中,按照病理科不同制片方法,也叫宮頸脫落細胞TCT制片法(英文ThinprepCytoiogicTesT)等。

                 為了準確的為患者提供檢查診斷結果,在試劑盒中有兩個部分尤為重要,其一是液基細胞標本保存液;其二是TCT的制片方法。

                液基細胞標本保存液主要特點是保持細胞原生態狀態;徹底分解粘液、紅細胞及雜質。液基細胞標本保存液使細胞得到完整的保存,讓病理工作者觀測到患者最真實地細胞情況。

             

             



             

                TCT制片方法最常用主要有手工法、TCT膜式法和離心沉降法三種。

                手工法是婦科醫生用取樣器在患者宮頸口取脫落細胞,然后打開康乃欣生產的液基細胞處理試劑盒,取出液基細胞保存液,旋開液基細胞保存液蓋,將病理取樣置液基細胞保存液中,蓋上液基細胞標本保存液蓋,將它放在液基細胞震蕩儀上震蕩5分鐘,目的是打散宮頸脫落細胞,使脫落細胞均勻分布在保存液中。取出液基細胞試劑盒中一次性吸管,在震蕩后的液基細胞保存液中吸取0.2-0.39毫升標本,平鋪在病理載玻片上,將載玻片平放在工作臺上,等待自然干后,進行染色(HE染色或巴氏染色),然后封片,鏡檢。

                TCT膜式法,早在19世紀中旬,這種方法是發達國家在宮頸癌篩查常用的一種方法,在改革開放后引入我國。TCT膜式法制片過程,是通過TCT膜式機制片。TCT膜式機的原理是通過負壓和正壓將附在TCT膜管上的細胞吹在載玻片上的制片方式。這種TCT制片方式簡單,快捷,缺陷是TCT膜管容易吹裂,制片一次一個。TCT膜管目前市場上很多廠家,國外有美國、英國、德國進口的,國內也有很多廠家。質量最可靠的是美國的。英國、德國的TCT膜管易裂,膜網不均,國產的質量更次,會導致病理脫落細胞片中細胞量少,嚴重降低宮頸癌陽性篩查率。

                離心沉降法,首先打開康乃欣液基細胞處理試劑盒,拿出液基細胞標本保存液、宮頸脫落細胞取樣器、一次性吸管等。將脫落細胞取樣器取樣,旋開液基細胞標本保存液蓋,放入宮頸脫落細胞,蓋上蓋。將液基細胞標本保存液放置液基細胞震蕩儀上震蕩4分鐘,使液基細胞處理液充分處理病理標本,充分打散宮頸脫落細胞。用一次吸管吸取液基細胞標本處理液1ml-2.5ml放入康乃欣專供制片夾中。將編好號碼的制片夾放入康乃欣液基細胞制片機(也叫康乃欣液基細胞薄層制片機),將液基細胞制片機通電,時間調到4-5分鐘,轉速設為1200-1500轉/秒。啟動液基細胞制片機,聽到“咔”的一聲。告知液基制片已經制作完成,最后染色鏡檢。

               針對以上三種制片方法,中山市康乃欣生物醫療公司科技公司誠意為廣大醫療機構打造最好的液基細胞處理試劑盒,TCT膜管采用的是美國進口膜,TCT膜網均勻,彈力足。TCT離心沉降法是康乃欣生物醫療科技公司最成熟的制片方法,制作效果可與美國進口自動沉降制片一體機制的制片可媲美。

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